We use both cell culture and animal models. Studies of patient samples collected at the Danish Center for Sleep Medicine translate basic findings to the clinical situation.
SINGLE CELL SEQUENCINGTo fully understand the regulation and function of certain neurons, these need to be isolated from other neuronal subpopulations as well as from glial and endothelial cells. The last decade has seen tremendous progress in the development of tools for targeting and examining neuronal subpopulations. We wish have established single neuron specific RNA sequencing. This allows us to map the transcriptome of hcrt neurons during immune activation and identify molecules of importance to neuronal vulnerability and survival. We use the 10x Genomics technology.